miR160 microRNA precursor family
| miR160 microRNA precursor family | |
|---|---|
Predicted secondary structure and sequence conservation of miR160 | |
| Identifiers | |
| Symbol | miR160 |
| Rfam | RF00247 |
| miRBase | MI0000190 |
| miRBase family | MIPF0000032 |
| Other data | |
| RNA type | Gene; miRNA |
| Domain | Eukaryota |
| GO | GO:0035195 GO:0035068 |
| SO | SO:0001244 |
| PDB structures | PDBe |
In molecular biology, miR160 is a microRNA that has been predicted or experimentally confirmed in a range of plant species including Arabidopsis thaliana (mouse-ear cress) and Oryza sativa (rice).[1] miR-160 is predicted to bind complementary sites in the untranslated regions of auxin response factor genes to regulate their expression.[2] The hairpin precursors (represented here) are predicted based on base pairing and cross-species conservation; their extents are not known. In this case, the mature sequence is excised from the 5' arm of the hairpin.
Specifically, three of the 23 Arabidopsis thaliana AUXIN RESPONSE FACTOR (ARF) genes—ARF10, ARF16, and ARF17—are post-transcriptionally regulated by miR160.[3][4] Experimental evidence demonstrates that miR160-mediated regulation of these transcription factors is required for normal plant development.[3]
When ARF17 is engineered to be resistant to miR160-mediated cleavage, plants exhibit multiple developmental defects, demonstrating the importance of miR160 regulation of auxin response pathways.[3] Similar experiments with another target, ARF10, showed that repression by miR160 is required for proper seed germination and post-embryonic development.[5]
References
- ^ "miRNA gene family: MIR160". mirBASE. University of Manchester. Retrieved 5 September 2011.
{{cite web}}: CS1 maint: deprecated archival service (link) - ^ Rhoades MW, Reinhart BJ, Lim LP, Burge CB, Bartel B, Bartel DP (August 2002). "Prediction of plant microRNA targets". Cell. 110 (4): 513–20. doi:10.1016/S0092-8674(02)00863-2. PMID 12202040.
- ^ a b c Mallory AC, Bartel DP, Bartel B (May 2005). "MicroRNA-directed regulation of Arabidopsis AUXIN RESPONSE FACTOR17 is essential for proper development and modulates expression of early auxin response genes". The Plant Cell. 17 (5): 1360–75. doi:10.1105/tpc.105.031716. PMC 1091760. PMID 15829600.
- ^ Liu X, Huang J, Wang Y, Khanna K, Xie Z, Owen HA, Zhao D (May 2010). "The role of floral organs in carpels, an Arabidopsis loss-of-function mutation in MicroRNA160a, in organogenesis and the mechanism regulating its expression". The Plant Journal. 62 (3): 416–28. doi:10.1111/j.1365-313X.2010.04164.x. PMID 20136729.
- ^ Liu PP, Montgomery TA, Fahlgren N, Kasschau KD, Nonogaki H, Carrington JC (October 2007). "Repression of AUXIN RESPONSE FACTOR10 by microRNA160 is critical for seed germination and post-germination stages". The Plant Journal. 52 (1): 133–46. doi:10.1111/j.1365-313X.2007.03218.x. PMID 17672844.
External links