Cucurbitacin Delta23-reductase
| cucurbitacin delta23-reductase | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Identifiers | |||||||||
| EC no. | 1.3.1.5 | ||||||||
| CAS no. | 37256-38-5 | ||||||||
| Databases | |||||||||
| IntEnz | IntEnz view | ||||||||
| BRENDA | BRENDA entry | ||||||||
| ExPASy | NiceZyme view | ||||||||
| KEGG | KEGG entry | ||||||||
| MetaCyc | metabolic pathway | ||||||||
| PRIAM | profile | ||||||||
| PDB structures | RCSB PDB PDBe PDBsum | ||||||||
| Gene Ontology | AmiGO / QuickGO | ||||||||
| |||||||||
In enzymology, cucurbitacin Delta23-reductase (EC 1.3.1.5) is an enzyme that catalyzes the chemical reaction
The three substrates of this enzyme are the plant steroid curbitacin b, reduced nicotinamide adenine dinucleotide (NADH), and a proton. Its products are dihydrocucurbitacin b, and oxidised NAD+. The enzyme can use nicotinamide adenine dinucleotide phosphate as an alternative cofactor.[1][2][3]
This enzyme belongs to the family of oxidoreductases, specifically those acting on the CH-CH group of donor with NAD+ or NADP+ as acceptor. The systematic name of this enzyme class is 23,24-dihydrocucurbitacin:NAD(P)+ Delta23-oxidoreductase. This enzyme is also called NAD(P)H: cucurbitacin B Delta23-oxidoreductase. It contains manganese.[1]
References
- ^ a b Enzyme 1.3.1.5 at KEGG Pathway Database.
- ^ Schabort JC, Potgieter DJ (1968). "Cucurbitacin B delta 23-reductase from Cucurbita maxima. II Cofactor requirements, enzyme kinetics, substrate specificity and other characteristics". Biochim. Biophys. Acta. 151 (1): 47–53. doi:10.1016/0005-2744(68)90159-9. PMID 4384331.
- ^ Schabort JC, Potgieter DJ, de Villiers V (1968). "Cucurbitacin B delta 23-reductase from Cucurbita maxima. I. Assay methods, isolation and purification". Biochim. Biophys. Acta. 151 (1): 33–46. doi:10.1016/0005-2744(68)90158-7. PMID 5640163.