Uroporphyrinogen-III C-methyltransferase

Uroporphyrinogen-III C-methyltransferase
Identifiers
EC no.2.1.1.107
CAS no.125752-76-3
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BRENDABRENDA entry
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NCBIproteins

Uroporphyrinogen-III C-methyltransferase (EC 2.1.1.107), uroporphyrinogen methyltransferase, uroporphyrinogen-III methyltransferase, adenosylmethionine-uroporphyrinogen III methyltransferase, S-adenosyl-L-methionine-dependent uroporphyrinogen III methylase, uroporphyrinogen-III methylase, SirA, CysG, CobA, uroporphyrin-III C-methyltransferase, S-adenosyl-L-methionine:uroporphyrin-III C-methyltransferase) is an enzyme with systematic name S-adenosyl-L-methionine:uroporphyrinogen-III C-methyltransferase.[1][2][3] This enzyme catalyses the following overall chemical reaction

+ 2 SAM
 
 
 
 
 
 
 
+ 2 SAH
 

The enzyme catalyses two methylation reactions. The first reaction converts uroporphyrinogen III into precorrin-1 and the second forms dihydrosirohydrochlorin (precorrin-2). In both cases the methyl group comes from the cofactor, S-adenosyl methionine (SAM), which loses its methyl group and becomes S-adenosyl-L-homocysteine (SAH).[4] These reactions are part of the biosynthetic pathway to cobalamin (vitamin B12) in both anaerobic and aerobic bacteria.[5]

See also

References

  1. ^ Warren MJ, Gonzalez MD, Williams HJ, Stolowich NJ, Scott AI (1990). "Uroporphyrinogen-III methylase catalyzes the enzymatic-synthesis of sirohydrochlorin-II and sirohydrochlorin-IV by a clockwise mechanism". J. Am. Chem. Soc. 112: 5343–5345. doi:10.1021/ja00169a048.
  2. ^ Warren MJ, Roessner CA, Santander PJ, Scott AI (February 1990). "The Escherichia coli cysG gene encodes S-adenosylmethionine-dependent uroporphyrinogen III methylase". The Biochemical Journal. 265 (3): 725–9. doi:10.1042/bj2650725. PMC 1133693. PMID 2407234.
  3. ^ Schubert HL, Raux E, Brindley AA, Leech HK, Wilson KS, Hill CP, Warren MJ (May 2002). "The structure of Saccharomyces cerevisiae Met8p, a bifunctional dehydrogenase and ferrochelatase". The EMBO Journal. 21 (9): 2068–75. doi:10.1093/emboj/21.9.2068. PMC 125995. PMID 11980703.
  4. ^ Enzyme 2.1.1.107 at KEGG Pathway Database.
  5. ^ Battersby, Alan R. (2000). "Tetrapyrroles: The pigments of life". Natural Product Reports. 17 (6): 507–526. doi:10.1039/b002635m. PMID 11152419.