Toluene dioxygenase
| toluene dioxygenase | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Identifiers | |||||||||
| EC no. | 1.14.12.11 | ||||||||
| CAS no. | 120038-36-0 | ||||||||
| Databases | |||||||||
| IntEnz | IntEnz view | ||||||||
| BRENDA | BRENDA entry | ||||||||
| ExPASy | NiceZyme view | ||||||||
| KEGG | KEGG entry | ||||||||
| MetaCyc | metabolic pathway | ||||||||
| PRIAM | profile | ||||||||
| PDB structures | RCSB PDB PDBe PDBsum | ||||||||
| Gene Ontology | AmiGO / QuickGO | ||||||||
| |||||||||
In enzymology, toluene dioxygenase (EC 1.14.12.11) is an enzyme that catalyzes the chemical reaction
The four substrates of this enzyme are toluene, reduced nicotinamide adenine dinucleotide (NADH), oxygen, and a proton. Its products are (1S,2R)-3-methylcyclohexa-3,5-diene-1,2-diol and reduced NAD+.[1][2][3]
This enzyme is an oxidoreductase, that uses molecular oxygen as oxidant and incorporates both its atoms into the starting material. The systematic name of this enzyme class is toluene,NADH:oxygen oxidoreductase (1,2-hydroxylating). This enzyme is also called toluene 2,3-dioxygenase. It is an iron–sulfur protein that uses flavin adenine dinucleotide as a cofactor and participates in toluene and xylene degradation.[1]
References
- ^ a b Enzyme 1.14.12.11 at KEGG Pathway Database.
- ^ Renganathan V (1989). "Possible involvement of toluene-2,3-dioxygenase in defluorination of 3-fluoro-substituted benzenes by toluene-degrading Pseudomonas sp strain T-12". Applied and Environmental Microbiology. 55 (2): 330–334. Bibcode:1989ApEnM..55..330R. doi:10.1128/aem.55.2.330-334.1989. PMC 184110. PMID 16347845.
- ^ Subramanian V, Liu TN, Yeh WK, Gibson DT (1979). "Toluene dioxygenase: purification of an iron-sulfur protein by affinity chromatography". Biochemical and Biophysical Research Communications. 91 (3): 1131–9. Bibcode:1979BBRC...91.1131S. doi:10.1016/0006-291X(79)91998-3. PMID 526270.