Salicylate 1-monooxygenase

Salicylate 1-monooxygenase
Identifiers
EC no.1.14.13.1
CAS no.9059-28-3
Databases
IntEnzIntEnz view
BRENDABRENDA entry
ExPASyNiceZyme view
KEGGKEGG entry
MetaCycmetabolic pathway
PRIAMprofile
PDB structuresRCSB PDB PDBe PDBsum
Gene OntologyAmiGO / QuickGO
Search
PMCarticles
PubMedarticles
NCBIproteins

Salicylate 1-monooxygenase (EC 1.14.13.1) is an enzyme that catalyzes the chemical reaction

+ NADH
 
 
O2 + H+
H2O
 
 
 
+ NAD+ + CO2
 

The four substrates of this enzyme are salicylic acid, reduced nicotinamide adenine dinucleotide (NADH), oxygen, and a proton, Its products are catechol, oxidised NAD+, water, and carbon dioxide.[1][2][3]

The enzyme is a flavin-containing monooxygenase that uses molecular oxygen as oxidant and incorporates one of its atoms into the starting material. This enzyme participates in metabolic pathways involving the degradation of naphthalene and anthracene and some of their derivatives. It uses flavin adenine dinucleotide as a cofactor.[4][5]

Nomenclature

The systematic name of this enzyme class is salicylate,NADH:oxygen oxidoreductase (1-hydroxylating, decarboxylating).

Other names in common use include:

  • salicylate hydroxylase,
  • salicylate 1-hydroxylase,
  • salicylate monooxygenase, and
  • salicylate hydroxylase (decarboxylating)

References

  1. ^ Enzyme 1.14.13.1 at KEGG Pathway Database.
  2. ^ Suzuki K, Takemori S, Katagiri M (1969). "Mechanism of the salicylate hydroxylase reaction. IV. Fluorimetric analysis of the complex formation". Biochim. Biophys. Acta. 191 (1): 77–85. doi:10.1016/0005-2744(69)90316-7. PMID 4390441.
  3. ^ Takemori S, Yasuda H, Mihara K, Suzuki K, Katagiri M (1969). "Mechanism of the salicylate hydroxylase reaction. II. The enzyme-substrate complex". Biochim. Biophys. Acta. 191 (1): 58–68. doi:10.1016/0005-2744(69)90314-3. PMID 4898626.
  4. ^ Takemori S, Yasuda H, Mihara K, Suzuki K, Katagiri M (1969). "Mechanism of the salicylate hydroxylase reaction. 3 Characterization and reactivity of chemically or photochemically reduced enzyme-flavin". Biochim. Biophys. Acta. 191 (1): 69–76. doi:10.1016/0005-2744(69)90315-5. PMID 4309912.
  5. ^ Yamamoto S, Katagiri M, Maeno H, Hayaishi O (1965). "Salicylate hydroxylase, a monooxygenase requiring flavin adenine dinucleotide". J. Biol. Chem. 240 (8): 3408–3413. doi:10.1016/S0021-9258(18)97232-7. PMID 14321380.