Precorrin-2 C20-methyltransferase

precorrin-2 C20-methyltransferase
Identifiers
EC no.2.1.1.130
Databases
IntEnzIntEnz view
BRENDABRENDA entry
ExPASyNiceZyme view
KEGGKEGG entry
MetaCycmetabolic pathway
PRIAMprofile
PDB structuresRCSB PDB PDBe PDBsum
Gene OntologyAmiGO / QuickGO
Search
PMCarticles
PubMedarticles
NCBIproteins

In enzymology, precorrin-2 C20-methyltransferase (EC 2.1.1.130) is an enzyme that catalyzes the chemical reaction

+ SAM
 
 
 
 
 
 
 
precorrin-3A
+ SAH
 

This methylation reaction converts precorrin-2 into precorrin-3A. The methyl group comes from the cofactor, S-adenosyl methionine (SAM), which gives S-adenosyl-L-homocysteine (SAH).[1][2]

The enzyme belongs to the family of transferases, specifically those transferring one-carbon group methyltransferases. The systematic name of this enzyme class is S-adenosyl-L-methionine:precorrin-4 C20-methyltransferase and another names in common use is CobI. It is part of the biosynthetic pathway to cobalamin (vitamin B12) in aerobic bacteria.[3]

Structural studies

As of late 2007, two structures have been solved for this class of enzymes, with PDB accession codes 2E0K and 2E0N.

References

  1. ^ Enzyme 2.1.1.130 at KEGG Pathway Database.
  2. ^ Debussche L, Thibaut D, Cameron B, Crouzet J, Blanche F (1993). "Biosynthesis of the corrin macrocycle of coenzyme B12 in Pseudomonas denitrificans". J. Bacteriol. 175 (22): 7430–40. doi:10.1128/jb.175.22.7430-7440.1993. PMC 206888. PMID 8226690.
  3. ^ Battersby, Alan R. (2000). "Tetrapyrroles: The pigments of life: A Millennium review". Natural Product Reports. 17 (6): 507–526. doi:10.1039/B002635M. PMID 11152419.