Phthalate 4,5-dioxygenase

Phthalate 4,5-dioxygenase
Identifiers
EC no.1.14.12.7
CAS no.63626-44-8
Databases
IntEnzIntEnz view
BRENDABRENDA entry
ExPASyNiceZyme view
KEGGKEGG entry
MetaCycmetabolic pathway
PRIAMprofile
PDB structuresRCSB PDB PDBe PDBsum
Gene OntologyAmiGO / QuickGO
Search
PMCarticles
PubMedarticles
NCBIproteins

Phthalate 4,5-dioxygenase (EC 1.14.12.7) is an enzyme that catalyzes the chemical reaction

+ NADH
 
 
O2 + H+
 
 
 
 
cis-4,5-dihydroxycyclohexa-2,6-diene-1,2-dicarboxylic acid
+ NAD+
 

The four substrates of this enzyme are phthalic acid, nicotinamide adenine dinucleotide (NADH), oxygen, and a proton. Its products are cis-4,5-dihydroxycyclohexa-2,6-diene-1,2-dicarboxylic acid and NAD+.[1][2]

This enzyme belongs to the family of oxidoreductases, specifically those acting on paired donors, with molecular oxygen as oxidant and incorporation or reduction of oxygen. The systematic name of its class is phthalate,NADH:oxygen oxidoreductase (4,5-hydroxylating). Other names in common use include PDO, and phthalate dioxygenase. The enzyme participates in 2,4-dichlorobenzoate degradation. It is an Iron-sulfur protein which uses flavin mononucleotide as a cofactor.[1]

References

  1. ^ a b Enzyme 1.14.12.7 at KEGG Pathway Database.
  2. ^ Batie CJ, LaHaie E, Ballou DP (1987). "Purification and characterization of phthalate oxygenase and phthalate oxygenase reductase from Pseudomonas cepacia". J. Biol. Chem. 262 (4): 1510–8. doi:10.1016/S0021-9258(19)75664-6. PMID 3805038.