Pentalenolactone synthase

Pentalenolactone synthase
Identifiers
EC no.1.14.19.8
Databases
IntEnzIntEnz view
BRENDABRENDA entry
ExPASyNiceZyme view
KEGGKEGG entry
MetaCycmetabolic pathway
PRIAMprofile
PDB structuresRCSB PDB PDBe PDBsum
Search
PMCarticles
PubMedarticles
NCBIproteins

Pentalenolactone synthase (EC 1.14.19.8, Formerly EC 1.3.7.10, penM (gene), pntM (gene)) is an enzyme with systematic name pentalenolactone-F:oxidized-ferredoxin oxidoreductase (pentalenolactone forming). It catalyses the rearrangement reaction

pentalenolactone F
+ 2 H+ + 2 reduced
ferredoxin
 
 
O2
2 H2O
O2
2 H2O
 
pentalenolactone
+ 2 oxidised
ferredoxin
 

This is heme-thiolate protein (P-450), which is isolated from the bacteria Streptomyces exfoliatus and Streptomyces arenae.The transformation is the final step in the biosynthesis of pentalenolactone.[1][2][3]

References

  1. ^ Enzyme 1.14.19.8 at KEGG Pathway Database.
  2. ^ Cane, David E.; Oliver, John S.; Harrison, Paul H. M.; Abell, Christopher; Hubbard, Brian R.; Kane, Charles T.; Lattman, Rene (1990). "Biosynthesis of pentalenene and pentalenolactone". Journal of the American Chemical Society. 112 (11): 4513–4524. doi:10.1021/ja00167a059.
  3. ^ Zhu D, Seo MJ, Ikeda H, Cane DE (February 2011). "Genome mining in streptomyces. Discovery of an unprecedented P450-catalyzed oxidative rearrangement that is the final step in the biosynthesis of pentalenolactone". Journal of the American Chemical Society. 133 (7): 2128–31. Bibcode:2011JAChS.133.2128Z. doi:10.1021/ja111279h. PMC 3041837. PMID 21284395.