Orcinol 2-monooxygenase

orcinol 2-monooxygenase
Identifiers
EC no.1.14.13.6
CAS no.37217-34-8
Databases
IntEnzIntEnz view
BRENDABRENDA entry
ExPASyNiceZyme view
KEGGKEGG entry
MetaCycmetabolic pathway
PRIAMprofile
PDB structuresRCSB PDB PDBe PDBsum
Gene OntologyAmiGO / QuickGO
Search
PMCarticles
PubMedarticles
NCBIproteins

Orcinol 2-monooxygenase (EC 1.14.13.6) is an enzyme that catalyzes the chemical reaction

+ NADH
 
 
O2 + H+
H2O
 
 
 
2,3,5-trihydroxytoluene
+ NAD+
 

The four substrates of this enzyme are orcinol, reduced nicotinamide adenine dinucleotide (NADH), oxygen, and a proton. Its products are 2,3,5-trihydroxytoluene, oxidised NAD+, and water.[1][2]

The enzyme is a flavin-containing monooxygenase that uses molecular oxygen as oxidant and incorporates one of its atoms into the starting material. The systematic name of this enzyme class is orcinol,NADH:oxygen oxidoreductase (2-hydroxylating). It is also called orcinol hydroxylase. It uses flavin adenine dinucleotide as a cofactor.[1][3]

References

  1. ^ a b Enzyme 1.14.13.6 at KEGG Pathway Database.
  2. ^ Otha Y, Ribbons DW (1970). "Crystallization of orcinol hydroxylase from Pseudomonas putida". FEBS Lett. 11 (3): 189–192. Bibcode:1970FEBSL..11..189O. doi:10.1016/0014-5793(70)80525-7. PMID 11945483.
  3. ^ Montersino, Stefania; Tischler, Dirk; Gassner, George T.; Van Berkel, Willem J. H. (2011). "Catalytic and Structural Features of Flavoprotein Hydroxylases and Epoxidases". Advanced Synthesis & Catalysis. 353 (13): 2301–2319. doi:10.1002/adsc.201100384.