Leukotriene-B4 20-monooxygenase
| Leukotriene-B4 20-monooxygenase | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Identifiers | |||||||||
| EC no. | 1.14.14.94 | ||||||||
| CAS no. | 90119-11-2 | ||||||||
| Databases | |||||||||
| IntEnz | IntEnz view | ||||||||
| BRENDA | BRENDA entry | ||||||||
| ExPASy | NiceZyme view | ||||||||
| KEGG | KEGG entry | ||||||||
| MetaCyc | metabolic pathway | ||||||||
| PRIAM | profile | ||||||||
| PDB structures | RCSB PDB PDBe PDBsum | ||||||||
| Gene Ontology | AmiGO / QuickGO | ||||||||
| |||||||||
Leukotriene-B4 20-monooxygenase (EC 1.14.14.94) is an enzyme that catalyzes the chemical reaction
The four substrates of this enzyme are leukotriene B4, reduced nicotinamide adenine dinucleotide phosphate (NADPH), oxygen and a proton. Its products are 20-hydroxy-leukotriene B4, oxidised NADP+, and water.[1][2][3][4]
This enzyme is a cytochrome P450 omega hydroxylase that uses molecular oxygen as oxidant and incorporates one of its atoms into the starting material. The systematic name of this enzyme class is (6Z,8E,10E,14Z)-(5S,12R)-5,12-dihydroxyicosa-6,8,10,14-tetraenoate,N ADPH:oxygen oxidoreductase (20-hydroxylating). Other names in common use include leukotriene-B4 20-hydroxylase, leucotriene-B4 omega-hydroxylase, LTB4 20-hydroxylase, and LTB4 omega-hydroxylase. It participates in arachidonic acid metabolism and contains heme.[1]
References
- ^ a b Enzyme 1.14.14.94 at KEGG Pathway Database.
- ^ Romano MC, Eckardt RD, Bender PE, Leonard TB, Straub KM, Newton JF (1987). "Biochemical characterization of hepatic microsomal leukotriene B4 hydroxylases". J. Biol. Chem. 262 (4): 1590–5. doi:10.1016/S0021-9258(19)75676-2. PMID 3027095.
- ^ Shak S, Goldstein IM (1985). "Leukotriene B4 omega-hydroxylase in human polymorphonuclear leukocytes. Partial purification and identification as a cytochrome P-450". J. Clin. Invest. 76 (3): 1218–28. doi:10.1172/JCI112077. PMC 424026. PMID 4044832.
- ^ Soberman RJ, Harper TW, Murphy RC, Austen KF (1985). "Identification and functional characterization of leukotriene B4 20-hydroxylase of human polymorphonuclear leukocytes". Proc. Natl. Acad. Sci. U.S.A. 82 (8): 2292–5. doi:10.1073/pnas.82.8.2292. PMC 397543. PMID 2986111.