Dimethylglycine oxidase

Dimethylglycine oxidase
Identifiers
EC no.1.5.3.10
CAS no.74870-79-4
Databases
IntEnzIntEnz view
BRENDABRENDA entry
ExPASyNiceZyme view
KEGGKEGG entry
MetaCycmetabolic pathway
PRIAMprofile
PDB structuresRCSB PDB PDBe PDBsum
Gene OntologyAmiGO / QuickGO
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Dimethylglycine oxidase (EC 1.5.3.10) is an enzyme that catalyzes the chemical reaction

+ H2O
 
 
O2
H2O2
 
 
 
 

The three substrates of this enzyme are dimethylglycine, water and oxygen. Its products are sarcosine, hydrogen peroxide, and formaldehyde.[1] The enzyme does not further demethylate the product sarcosine to glycine.[2] Enzymes such as sarcosine oxidase are known which can do that conversion.[3]

This enzyme belongs to the family of oxidoreductases, specifically those acting on the CH-NH group of donors with oxygen as acceptor. The systematic name of this enzyme class is N,N-dimethylglycine:oxygen oxidoreductase (demethylating). It employs one cofactor, FAD.

Structural studies

As of late 2007, 3 structures have been solved for this class of enzymes, with PDB accession codes 1PJ5​, 1PJ6​, and 1PJ7​.

References

  1. ^ Enzyme 1.5.3.10 at KEGG Pathway Database.
  2. ^ Mori, Nobuhiro; Kawakami, Bunsei; Tani, Yoshiki; Yamada, Hideaki (1980). "Purification and properties of dimethylglycine oxidase from Cylindrocarpon didymum M-1". Agricultural and Biological Chemistry. 44 (6): 1383–1389. doi:10.1271/bbb1961.44.1383.
  3. ^ Suzuki, H. (1994). "Sarcosine oxidase: Structure, function, and the application to creatinine determination". Amino Acids. 7 (1): 27–43. doi:10.1007/BF00808444. PMID 24185971.