Deacetylcephalosporin-C acetyltransferase
| Deacetylcephalosporin-C acetyltransferase | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Identifiers | |||||||||
| EC no. | 2.3.1.175 | ||||||||
| Databases | |||||||||
| IntEnz | IntEnz view | ||||||||
| BRENDA | BRENDA entry | ||||||||
| ExPASy | NiceZyme view | ||||||||
| KEGG | KEGG entry | ||||||||
| MetaCyc | metabolic pathway | ||||||||
| PRIAM | profile | ||||||||
| PDB structures | RCSB PDB PDBe PDBsum | ||||||||
| |||||||||
Deacetylcephalosporin-C acetyltransferase (EC 2.3.1.175) is an enzyme that catalyzes the chemical reaction
The two substrates of this enzyme are deacetylcephalosporin C and acetyl coenzyme A. Its products are cephalosporin C and coenzyme A.[1][2][3][4]
This enzyme belongs to the family of transferases, specifically those acyltransferases transferring groups other than aminoacyl groups. The systematic name of this enzyme class is acetyl-CoA:deacetylcephalosporin-C O-acetyltransferase. Other names in common use include acetyl-CoA:deacetylcephalosporin-C acetyltransferase, DAC acetyltransferase, cefG, deacetylcephalosporin C acetyltransferase, acetyl coenzyme A:DAC acetyltransferase, acetyl-CoA:DAC acetyltransferase, CPC acetylhydrolase, acetyl-CoA:DAC O-acetyltransferase, and DAC-AT. This enzyme catalyses the final step in the conversion of penicillin N to cephalosporin C.[5][6] The industrial production of cephalosporin C in Acremonium chrysogenum has led to optimisation of this enzyme pathway.[7]
References
- ^ Enzyme 2.3.1.175 at KEGG Pathway Database.
- ^ Gutierrez S, Velasco J, Fernandez FJ, Martin JF (1992). "The cefG gene of Cephalosporium acremonium is linked to the cefEF gene and encodes a deacetylcephalosporin C acetyltransferase closely related to homoserine O-acetyltransferase". J. Bacteriol. 174 (9): 3056–64. doi:10.1128/jb.174.9.3056-3064.1992. PMC 205961. PMID 1569032.
- ^ K; Sugiura, H; Matsuyama, K; Matsumoto, H; Ichikawa, S; Komatsu, K (1992). "Cloning and disruption of the cefG gene encoding acetyl coenzyme A: deacetylcephalosporin C o-acetyltransferase from Acremonium chrysogenum". Biochem. Biophys. Res. Commun. 186 (1): 40–6. Bibcode:1992BBRC..186...40M. doi:10.1016/S0006-291X(05)80772-7. PMID 1632779.
- ^ JL, Diez B, Martin JF (1997). "Expression of the cefG gene is limiting for cephalosporin biosynthesis in Acremonium chrysogenum". Appl. Microbiol. Biotechnol. 48 (5): 606–14. doi:10.1007/s002530051103. PMID 9421924.
- ^ Velasco J, Gutierrez S, Campoy S, Martin JF (1999). "Molecular characterization of the Acremonium chrysogenum cefG gene product: the native deacetylcephalosporin C acetyltransferase is not processed into subunits". Biochem. J. 337 (3): 379–85. doi:10.1042/0264-6021:3370379. PMC 1219988. PMID 9895280.
- ^ AT, Kosalkova K; Gutiérrez, S; Fernández, FJ; Velasco, J; Fierro, F; Marcos, AT; Kosalkova, K (1994). "Expression of genes and processing of enzymes for the biosynthesis of penicillins and cephalosporins". Antonie van Leeuwenhoek. 65 (3): 227–43. doi:10.1007/BF00871951. PMID 7847890.
- ^ Liu, Ling; Chen, Zhen; Liu, Wuyi; et al. (2022). "Cephalosporin C biosynthesis and fermentation in Acremonium chrysogenum". Applied Microbiology and Biotechnology. 106 (19–20): 6413–6426. doi:10.1007/s00253-022-12181-w. PMID 36114850.