D-nopaline dehydrogenase

D-nopaline dehydrogenase
Identifiers
EC no.1.5.1.19
CAS no.64763-57-1
Databases
IntEnzIntEnz view
BRENDABRENDA entry
ExPASyNiceZyme view
KEGGKEGG entry
MetaCycmetabolic pathway
PRIAMprofile
PDB structuresRCSB PDB PDBe PDBsum
Gene OntologyAmiGO / QuickGO
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PMCarticles
PubMedarticles
NCBIproteins

In enzymology, D-nopaline dehydrogenase (EC 1.5.1.19) is an enzyme that catalyzes the chemical reaction

 
 
H2O
H+
H2O
H+
 
+ NADPH +
 

The three substrates of this enzyme are nopaline, oxidised nicotinamide adenine dinucleotide phosphate (NADP+), and water. Its products are L-arginine, reduced NADPH, α-ketoglutaric acid and a proton.[1] Nopaline is a member of the class of compounds called opines.[2]

This enzyme belongs to the family of oxidoreductases, specifically those acting on the CH-NH group of donors with NAD+ or NADP+ as acceptor. The systematic name of this enzyme class is N2-(D-1,3-dicarboxypropyl)-L-arginine:NADP+ oxidoreductase (L-arginine-forming). Other names in common use include D-nopaline synthase, nopaline dehydrogenase, nopaline synthase, NOS, 2-N-(D-1,3-dicarboxypropyl)-L-arginine:NADP+ oxidoreductase, and (L-arginine-forming). This enzyme participates in arginine and proline metabolism.

References

  1. ^ Enzyme 1.5.1.19 at KEGG Pathway Database.
  2. ^ Kemp JD, Sutton DW, Hack E (1979). "Purification and characterization of the crown gall specific enzyme nopaline synthase". Biochemistry. 18 (17): 3755–60. doi:10.1021/bi00584a017. PMID 476084.