D-lysopine dehydrogenase

D-lysopine dehydrogenase
Identifiers
EC no.1.5.1.16
CAS no.65187-41-9
Databases
IntEnzIntEnz view
BRENDABRENDA entry
ExPASyNiceZyme view
KEGGKEGG entry
MetaCycmetabolic pathway
PRIAMprofile
PDB structuresRCSB PDB PDBe PDBsum
Gene OntologyAmiGO / QuickGO
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PMCarticles
PubMedarticles
NCBIproteins

In enzymology, D-lysopine dehydrogenase (EC 1.5.1.16) is an enzyme that catalyzes the chemical reaction

D-lysopine
 
 
H2O
H+
H2O
H+
 
+ NADPH +
 

The three substrates of this enzyme are D-lysopine, oxidised nicotinamide adenine dinucleotide phosphate (NADP+), and water. Its products are L-lysine, reduced NADPH, pyruvic acid, and a proton.[1][2]

This enzyme belongs to the family of oxidoreductases, specifically those acting on the CH-NH group of donors with NAD+ or NADP+ as acceptor. The systematic name of this enzyme class is N2-(D-1-carboxyethyl)-L-lysine:NADP+ oxidoreductase (L-lysine-forming). Other names in common use include D-lysopine synthase, lysopine dehydrogenase, D(+)-lysopine dehydrogenase, 2-N-(D-1-carboxyethyl)-L-lysine:NADP+ oxidoreductase, and (L-lysine-forming). This enzyme participates in lysine degradation.

D-lysopine is an opine, a compound found in plant crown gall tumors or hairy root tumors produced by pathogenic bacteria of the genus Agrobacterium and Rhizobium.[3]

References

  1. ^ Enzyme 1.5.1.16 at KEGG Pathway Database.
  2. ^ Otten LA, Vreugdenhil D, Schilperoort RA (1977). "Properties of D(+)-lysopine dehydrogenase from crown gall tumour tissue". Biochim. Biophys. Acta. 485 (2): 268–77. doi:10.1016/0005-2744(77)90163-2. PMID 21695.
  3. ^ Moore, Larry W.; Chilton, William Scott; Canfield, Marilyn L. (1997). "Diversity of opines and opine-catabolizing bacteria isolated from naturally occurring crown gall tumors". Applied and Environmental Microbiology. 63 (1): 201–207. Bibcode:1997ApEnM..63..201M. doi:10.1128/AEM.63.1.201-207.1997. PMC 1389099. PMID 16535484.