Anthranilate 1,2-dioxygenase (deaminating, decarboxylating)

Anthranilate 1,2-dioxygenase (deaminating, decarboxylating)
Identifiers
EC no.1.14.12.1
CAS no.9059-17-0
Databases
IntEnzIntEnz view
BRENDABRENDA entry
ExPASyNiceZyme view
KEGGKEGG entry
MetaCycmetabolic pathway
PRIAMprofile
PDB structuresRCSB PDB PDBe PDBsum
Gene OntologyAmiGO / QuickGO
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PMCarticles
PubMedarticles
NCBIproteins

Anthranilate 1,2-dioxygenase (deaminating, decarboxylating) (EC 1.14.12.1) is an enzyme that catalyzes the chemical reaction

+ NADH + H+
 
 
O2
CO2
 
 
 
+ NAD+ + NH3
 

The four substrates of this enzyme are anthranilic acid, reduced nicotinamide adenine dinucleotide (NADH), oxygen, and a proton. Its products are catechol, carbon dioxide, oxidised NAD+, and ammonia. The enzyme can also use nicotinamide adenine dinucleotide phosphate as a cofactor.[1][2]

This enzyme is an oxidoreductase which uses molecular oxygen as oxidant and incorporates its atoms into the product.[3] The systematic name of this enzyme class is anthranilate,NAD(P)H:oxygen oxidoreductase (1,2-hydroxylating, deaminating, decarboxylating). Other names in common use include anthranilate hydroxylase, anthranilic hydroxylase, and anthranilic acid hydroxylase. It participates in three metabolic pathways: benzoate degradation via hydroxylation, carbazole degradation, and nitrogen metabolism. It requires ferrous iron.[1]

References

  1. ^ a b Enzyme 1.14.12.1 at KEGG Pathway Database.
  2. ^ Taniuchi H, Hatanaka M, Kuno S, Hayaishi O, Nakajima M, Kurihara N (1964). "Enzymatic formation of catechol from anthranilic acid". J. Biol. Chem. 239 (7): 2204–2211. doi:10.1016/S0021-9258(20)82221-2. PMID 14209949.
  3. ^ Kobayashi S, Hayaishi O (1970). "Anthranilic acid conversion to catechol (Pseudomonas)". Metabolism of Amino Acids and Amines Part A. Methods Enzymol. Vol. 17A. pp. 505–510. doi:10.1016/0076-6879(71)17231-x. ISBN 978-0-12-181874-6.