4-sulfobenzoate 3,4-dioxygenase
| 4-sulfobenzoate 3,4-dioxygenase | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Identifiers | |||||||||
| EC no. | 1.14.12.8 | ||||||||
| CAS no. | 122933-81-7 | ||||||||
| Databases | |||||||||
| IntEnz | IntEnz view | ||||||||
| BRENDA | BRENDA entry | ||||||||
| ExPASy | NiceZyme view | ||||||||
| KEGG | KEGG entry | ||||||||
| MetaCyc | metabolic pathway | ||||||||
| PRIAM | profile | ||||||||
| PDB structures | RCSB PDB PDBe PDBsum | ||||||||
| Gene Ontology | AmiGO / QuickGO | ||||||||
| |||||||||
4-sulfobenzoate 3,4-dioxygenase (EC 1.14.12.8) is an enzyme originally obtained from Comamonas testosteroni that catalyzes the chemical reaction
The four substrates of this enzyme are 4-sulfobenzoic acid, reduced nicotinamide adenine dinucleotide (NADH), oxygen and a proton. Its products are protocatechuic acid (3,4-dihydroxybenzoic acid), NAD+, and sulfurous acid.[1][2]
This enzyme is an oxidoreductase, which uses molecular oxygen as oxidant and incorporates its atoms into the starting material. The systematic name of this enzyme class is 4-sulfobenzoate,NADH:oxygen oxidoreductase (3,4-hydroxylating, sulfite-forming). Other names in common use include 4-sulfobenzoate dioxygenase, and 4-sulfobenzoate 3,4-dioxygenase system. It participates in toluene degradation and is an iron–sulfur protein that uses flavin mononucleotide as a cofactor.[1]
References
- ^ a b Enzyme 1.14.12.8 at KEGG Pathway Database.
- ^ Locher HH, Leisinger T, Cook AM (March 1991). "4-Sulphobenzoate 3,4-dioxygenase. Purification and properties of a desulphonative two-component enzyme system from Comamonas testosteroni T-2". Biochem. J. 274 (3): 833–42. doi:10.1042/bj2740833. PMC 1149986. PMID 2012609.