4-hydroxyphenylacetate 1-monooxygenase

4-hydroxyphenylacetate 1-monooxygenase
Identifiers
EC no.1.14.13.18
CAS no.55326-44-8
Databases
IntEnzIntEnz view
BRENDABRENDA entry
ExPASyNiceZyme view
KEGGKEGG entry
MetaCycmetabolic pathway
PRIAMprofile
PDB structuresRCSB PDB PDBe PDBsum
Gene OntologyAmiGO / QuickGO
Search
PMCarticles
PubMedarticles
NCBIproteins

4-hydroxyphenylacetate 1-monooxygenase (EC 1.14.13.18) is an enzyme that catalyzes the chemical reaction

+ NADH + H+
 
 
O2
H2O
 
 
 
+ NAD+
 

The four substrates of this enzyme are 4-hydroxyphenylacetic acid, reduced nicotinamide adenine dinucleotide (NADH), oxygen and a proton. Its products are homogentisic acid, oxidised NAD+, and water. The enzyme can use nicotinamide adenine dinucleotide phosphate as an alternative cofactor.[1][2]

The enzyme is a flavin-containing monooxygenase that uses molecular oxygen as oxidant and incorporates one of its atoms into the starting material. The systematic name of this enzyme class is 4-hydroxyphenylacetate,NAD(P)H:oxygen oxidoreductase (1-hydroxylating). Other names in common use include 4-hydroxyphenylacetate 1-hydroxylase, 4-hydroxyphenylacetic 1-hydroxylase, and 4-HPA 1-hydroxylase. This enzyme participates in tyrosine metabolism.[1]

References

  1. ^ a b Enzyme 1.14.13.18 at KEGG Pathway Database.
  2. ^ Hareland, W. A.; Crawford, R. L.; Chapman, P. J.; Dagley, S. (1975). "Metabolic function and properties of 4-hydroxyphenylacetic acid 1-hydroxylase from Pseudomonas acidovorans". Journal of Bacteriology. 121 (1): 272–285. doi:10.1128/jb.121.1.272-285.1975. PMC 285641. PMID 234937.