4-chlorophenylacetate 3,4-dioxygenase

4-chlorophenylacetate 3,4-dioxygenase
Identifiers
EC no.1.14.12.9
CAS no.105006-00-6
Databases
IntEnzIntEnz view
BRENDABRENDA entry
ExPASyNiceZyme view
KEGGKEGG entry
MetaCycmetabolic pathway
PRIAMprofile
PDB structuresRCSB PDB PDBe PDBsum
Gene OntologyAmiGO / QuickGO
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PMCarticles
PubMedarticles
NCBIproteins

4-chlorophenylacetate 3,4-dioxygenase (EC 1.14.12.9) is an enzyme that catalyzes the chemical reaction

4-Chlorophenylacetic acid
+ NADH
 
 
O2 + H+
 
 
 
 
+ NAD+ + HCl
 

The four substrates of this enzyme are 4-chlorophenylacetic acid, reduced nicotinamide adenine dinucleotide (NADH), oxygen, and a proton. Its products are 3,4-dihydroxyphenylacetic acid, oxidised NAD+, and hydrochloric acid.[1][2]

This enzyme is an oxidoreductase, which uses molecular oxygen as oxidant and incorporates both its atoms into the starting material. The systematic name of the enzyme class is 4-chlorophenylacetate,NADH:oxygen oxidoreductase (3,4-hydroxylating, dechlorinating). It is an iron–sulfur protein that is part of tyrosine metabolism.[1]

References

  1. ^ a b Enzyme 1.14.12.9 at KEGG Pathway Database.
  2. ^ Markus A, Krekel D, Lingens F (1986). "Purification and some properties of component A of the 4-chlorophenylacetate 3,4-dioxygenase from Pseudomonas species strain CBS". J. Biol. Chem. 261 (27): 12883–8. doi:10.1016/S0021-9258(18)67175-3. PMID 3745216.