3-phenylpropanoate dioxygenase

3-phenylpropanoate dioxygenase
Identifiers
EC no.1.14.12.19
Databases
IntEnzIntEnz view
BRENDABRENDA entry
ExPASyNiceZyme view
KEGGKEGG entry
MetaCycmetabolic pathway
PRIAMprofile
PDB structuresRCSB PDB PDBe PDBsum
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NCBIproteins

3-phenylpropanoate dioxygenase (EC 1.14.12.19) is an enzyme that catalyzes the chemical reaction

+ NADH
 
 
O2 + H+
 
 
 
 
cis-diol product
+ NAD+
 

The four substrates of this enzyme are phenylpropanoic acid, reduced nicotinamide adenine dinucleotide (NADH), oxygen, and a proton. Its products are the cis-diol, 3-[(5S,6R)-5,6-dihydroxycyclohexa-1,3-dienyl]propanoic acid, and oxidised NAD+.[1][2][3]

This enzyme is an oxidoreductase that uses molecular oxygen as oxidant and incorporates both its atoms into the starting material. The systematic name of this enzyme class is 3-phenylpropanoate,NADH:oxygen oxidoreductase (2,3-hydroxylating). Other names in common use include HcaA1A2CD, Hca dioxygenase, and 3-phenylpropionate dioxygenase. It can catalyse the same reaction in related compounds, for example cinnamic acid.[1]

 
 
 
 
 
 
cis-diol product

References

  1. ^ a b Enzyme 1.14.12.19 at KEGG Pathway Database.
  2. ^ Diaz E, Ferrandez A, Garcia JL (1998). "Characterization of the hca cluster encoding the dioxygenolytic pathway for initial catabolism of 3-phenylpropionic acid in Escherichia coli K-12". J. Bacteriol. 180 (11): 2915–23. doi:10.1128/JB.180.11.2915-2923.1998. PMC 107259. PMID 9603882.
  3. ^ Burlingame R, Chapman PJ (1983). "Catabolism of phenylpropionic acid and its 3-hydroxy derivative by Escherichia coli". J. Bacteriol. 155 (1): 113–21. doi:10.1128/jb.155.1.113-121.1983. PMC 217659. PMID 6345502.