3-hydroxybenzoate 6-monooxygenase

3-hydroxybenzoate 6-monooxygenase
Identifiers
EC no.1.14.13.24
CAS no.51570-26-4
Databases
IntEnzIntEnz view
BRENDABRENDA entry
ExPASyNiceZyme view
KEGGKEGG entry
MetaCycmetabolic pathway
PRIAMprofile
PDB structuresRCSB PDB PDBe PDBsum
Gene OntologyAmiGO / QuickGO
Search
PMCarticles
PubMedarticles
NCBIproteins

3-hydroxybenzoate 6-monooxygenase (EC 1.14.13.24) is an enzyme that catalyzes the chemical reaction

+ NADH + H+
 
 
O2
H2O
 
 
 
+ NAD+
 

The four substrates of this enzyme are 3-hydroxybenzoic acid, reduced nicotinamide adenine dinucleotide (NADH), oxygen and a proton. Its products are gentisic acid, oxidised NAD+, and water.[1][2]

The enzyme is a flavin-containing monooxygenase that uses molecular oxygen as oxidant and incorporates one of its atoms into the starting material. The systematic name of this enzyme class is 3-hydroxybenzoate,NADH:oxygen oxidoreductase (6-hydroxylating). Other names in common use include 3-hydroxybenzoate 6-hydroxylase, m-hydroxybenzoate 6-hydroxylase, and 3-hydroxybenzoic acid-6-hydroxylase. It participates in benzoate degradation via hydroxylation and uses flavin adenine dinucleotide as a cofactor.[1]

References

  1. ^ a b Enzyme 1.14.13.24 at KEGG Pathway Database.
  2. ^ Groseclose EE, Ribbons DW, Hughes H (1973). "3-Hydroxybenzoate 6-hydroxylase from Pseudomonas aeruginosa". Biochem. Biophys. Res. Commun. 55 (3): 897–903. Bibcode:1973BBRC...55..897G. doi:10.1016/0006-291X(73)91228-X. PMID 4357436.