3-hydroxybenzoate 4-monooxygenase
| 3-hydroxybenzoate 4-monooxygenase | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Identifiers | |||||||||
| EC no. | 1.14.13.23 | ||||||||
| CAS no. | 37256-76-1 | ||||||||
| Databases | |||||||||
| IntEnz | IntEnz view | ||||||||
| BRENDA | BRENDA entry | ||||||||
| ExPASy | NiceZyme view | ||||||||
| KEGG | KEGG entry | ||||||||
| MetaCyc | metabolic pathway | ||||||||
| PRIAM | profile | ||||||||
| PDB structures | RCSB PDB PDBe PDBsum | ||||||||
| Gene Ontology | AmiGO / QuickGO | ||||||||
| |||||||||
3-hydroxybenzoate 4-monooxygenase (EC 1.14.13.23) is an enzyme that catalyzes the chemical reaction
The foursubstrates of this enzyme are 3-hydroxybenzoic acid, reduced nicotinamide adenine dinucleotide phosphate (NADPH), oxygen, and a proton. Its products are protocatechuic acid, oxidised NADP+, and water.[1][2][3]
The enzyme is a flavin-containing monooxygenase that uses molecular oxygen as oxidant and incorporates one of its atoms into the starting material. The systematic name of this enzyme class is 3-hydroxybenzoate,NADPH:oxygen oxidoreductase (4-hydroxylating). It is also called 3-hydroxybenzoate 4-hydroxylase. It participates in benzoate degradation via hydroxylation and uses flavin adenine dinucleotide as a cofactor.[1]
Structural studies
As of late 2007, two structures have been solved for this class of enzymes, with PDB accession codes 2DKH and 2DKI.
References
- ^ a b Enzyme 1.14.13.23 at KEGG Pathway Database.
- ^ Michalover JL, Ribbons DW, Hughes H (1973). "3-Hydroxybenzoate 4-hydroxylase from Pseudomonas testosteroni". Biochem. Biophys. Res. Commun. 55 (3): 888–96. Bibcode:1973BBRC...55..888M. doi:10.1016/0006-291X(73)91227-8. PMID 4148586.
- ^ Premkumar R, Rao PV, Sreeleela NS, Vaidyanathan CS (1969). "m-Hydroxybenzoic acid 4-hydroxylase from Aspergillus niger". Can. J. Biochem. 47 (8): 825–7. doi:10.1139/o69-129. PMID 4390252.