2-chlorobenzoate 1,2-dioxygenase

2-chlorobenzoate 1,2-dioxygenase
Identifiers
EC no.1.14.12.13
CAS no.125268-83-9
Databases
IntEnzIntEnz view
BRENDABRENDA entry
ExPASyNiceZyme view
KEGGKEGG entry
MetaCycmetabolic pathway
PRIAMprofile
PDB structuresRCSB PDB PDBe PDBsum
Gene OntologyAmiGO / QuickGO
Search
PMCarticles
PubMedarticles
NCBIproteins

2-chlorobenzoate 1,2-dioxygenase (EC 1.14.12.13) is an enzyme from the bacterium Burkholderia cepacia that catalyzes the chemical reaction

+ NADH
 
 
O2
CO2
 
 
 
+ NAD+ + Cl
 

The four substrates of this enzyme are 2-chlorobenzoic acid, reduced nicotinamide adenine dinucleotide (NADH), oxygen and a proton. Its products are catechol, carbon dioxide, oxidised NAD+, and a chloride ion.[1][2]

This enzyme is an oxidoreductase that uses molecular oxygen as oxidant and incorporates both its atoms into the starting material. The systematic name of its class is 2-chlorobenzoate,NADH:oxygen oxidoreductase (1,2-hydroxylating, dechlorinating, decarboxylating). This enzyme is also called 2-halobenzoate 1,2-dioxygenase. It can oxidise other 2-halo substituted benzoic acids.[1]

References

  1. ^ a b Enzyme 1.14.12.13 at KEGG Pathway Database.
  2. ^ Fetzner S, Muller R, Lingens F (1989). "Degradation of 2-chlorobenzoate by Pseudomonas cepacia 2CBS". Biol. Chem. Hoppe-Seyler. 370 (11): 1173–82. doi:10.1515/bchm3.1989.370.2.1173. PMID 2610934.