2,6-dihydroxypyridine 3-monooxygenase

2,6-dihydroxypyridine 3-monooxygenase
Identifiers
EC no.1.14.13.10
CAS no.39279-38-4
Databases
IntEnzIntEnz view
BRENDABRENDA entry
ExPASyNiceZyme view
KEGGKEGG entry
MetaCycmetabolic pathway
PRIAMprofile
PDB structuresRCSB PDB PDBe PDBsum
Gene OntologyAmiGO / QuickGO
Search
PMCarticles
PubMedarticles
NCBIproteins

2,6-dihydroxypyridine 3-monooxygenase (EC 1.14.13.10) is an enzyme that catalyzes the chemical reaction

+ NADH
 
 
O2 + H+
H2O
 
 
 
2,3,6-Trihydroxypyridine
+ NAD+
 

The four substrates of this enzyme are 2,6-dihydroxypyridine, reduced nicotinamide adenine dinucleotide (NADH), oxygen, and a proton. Its products are 2,3,6-trihydroxypyridine, oxidised NAD+, and water.[1][2][3]

The enzyme is a flavin-containing monooxygenase that uses molecular oxygen as oxidant and incorporates one of its atoms into the starting material. The systematic name of this enzyme class is 2,6-dihydroxypyridine,NADH:oxygen oxidoreductase (3-hydroxylating). This enzyme is also called 2,6-dihydroxypyridine oxidase.[1] It uses flavin adenine dinucleotide as a cofactor.[4]

References

  1. ^ a b Enzyme 1.14.13.10 at KEGG Pathway Database.
  2. ^ Holmes PE, Rittenberg SC (1972). "The bacterial oxidation of nicotine. VII. Partial purification and properties of 2,6-dihydroxypyridine oxidase". J. Biol. Chem. 247 (23): 7622–7. doi:10.1016/S0021-9258(19)44570-5. PMID 4344227.
  3. ^ Holmes PE, Rittenberg SC, Knackmuss HJ (1972). "The bacterial oxidation of nicotine. 8. Synthesis of 2,3,6-trihydroxypyridine and accumulation and partial characterization of the product of 2,6-dihydroxypyridine oxidation". J. Biol. Chem. 247 (23): 7628–33. doi:10.1016/S0021-9258(19)44571-7. PMID 4636328.
  4. ^ Montersino, Stefania; Tischler, Dirk; Gassner, George T.; Van Berkel, Willem J. H. (2011). "Catalytic and Structural Features of Flavoprotein Hydroxylases and Epoxidases". Advanced Synthesis & Catalysis. 353 (13): 2301–2319. doi:10.1002/adsc.201100384.